生物医学工程

生物医学工程是发展最快的研究领域之一，它融合了医学、生物技术和工程领域的最新理念，为例如细胞生长支撑间质，人造组织、植入式生物医学设备等领域设计各种各样的技术方案。

材料的表面特性及材料与组织之间的相互作用是预测生物材料在受体内生物相容性的关键因素，在设计可靠的植入体、模拟组织、骨骼取代物和其他生物材料时也至关重要。TESCAN 的扫描电子显微镜系统是表征生物材料最理想的设备，具有高分辨率，且利用 Wide Field Optics™ 模式可以非常容易的在低倍下实时导航样品位置。

一旦在扫描电镜下找到感兴趣区域，客户就可以容易地切换到 Depth 模式或 Resolution 模式，对样品进行高倍细节信息的观察。
所有的仪器都可以配备多种探测器和分析附件，如 EDS, EBSD 和 Raman，其中 Raman 可以分析生物材料结构和具体组成成分。

采用低电压或低真空模式可以观察未经喷镀处理和荷电严重的样品。借助我们专用的 FIB-SEM 双束系统，研究者还可以对材料定点切割并直接观察材料与组织的界面情况，以及进行 3D 重构。

负载银纳米颗粒的高分子微纤维

Investigation of cell spreading on bioceramic materials

In the field of current implantology, the conventional usage of titanium alloys is being replaced by ceramic materials. Bioceramics are made by sintering of the ceramic powders (e. g. zirconia or alumina powders) and they are characterized by excellent hardness and tribological properties. Zirconia ceramics are becoming prevalent among biomaterials used in dental implantology. The aim of this study was to investigate osteoblastic spreading in contact with various oxide ceramics. The spreading of the osteoblastic cells MG63 on the zirconia and alumina surfaces was observed using a MIRA3 FEG SEM in the low vacuum mode in order to evaluate the biocompatibility of these ceramic materials.

pdf – 3.4 MB

Spreading of the osteoblasts on the zirconia ceramics

Low temperature scanning electron microscopy for Life Sciences

Low temperature scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves sample fixation using either flash-freezing in a liquid nitrogen slush or high-pressure freezing. The frozen samples are then transferred under vacuum to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed in high vacuum environment.

pdf – 4.8 MB