微生物学

微生物生物膜在感染、疾病传播和抗生素抗药性等许多过程中扮演着重要的角色。

微生物污染是导致塑料导管、假体植入物和医疗器材等生物医学设备无法使用的最大原因。

使用扫描电镜对微生物表面高分辨成像观察，有助于更深度解明微生物群体的形态、细菌间的相互作用和生物膜的形成。
TESCAN 扫描电子显微镜为研究者提供了大景深和高分辨性能，以更好地获取微生物的形貌。

利用 TESCAN 电镜的低真空 UniVac 模式或冷冻扫描电镜的环境模拟技术，可以直接观察微生物的生物膜，无需喷镀处理。
TESCAN MAIA3 是专为低加速电压下亚纳米分辨率设计的超高分辨扫描电镜，可以帮助科学家观察非镀膜的细菌个体的超微形貌特征。

变形链球菌

Adhesion of Streptococcus mutans to Dental Restorative Materials

Development of early childhood caries (ECC) is associated with the colonization of the oral cavity by oral microbes. The disease is characterized by severe decay of infant’s teeth. The colonization starts soon after the birth and its diversity increases later on, with the eruption of primary teeth. While external factors such as feeding habits, sugar expose and hereditary factors support development of the ECC, the onset and progression also greatly depends on the composition of microflora in the mouth cavity.

pdf – 1.1 MB

STEM detector in life science applications

Scanning transmission electron microscopy (STEM) has become a highly effective, easy-to-use technique for imaging biological thin sections (lamellae) in SEM. Multiple sample observation, automated stage navigation, and ultra-high resolution imaging make this technique an attractive solution for high-contrast observation of TEM sections with excellent results, and minimal operator’s time.

pdf – 2.4 MB

Low temperature scanning electron microscopy for Life Sciences

Low temperature scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves sample fixation using either flash-freezing in a liquid nitrogen slush or high-pressure freezing. The frozen samples are then transferred under vacuum to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed in high vacuum environment.

pdf – 4.8 MB

LIVE CELL IMAGING

Q-PHASE is a unique instrument dedicated to live cell imaging where advantages such as no need for labeling, low phototoxicity, easy segmentation, cell dry mass interpretation of measured signal, and suitability for long term experiments are used. Q-PHASE is built as a transmitted light microscope in an inverted configuration for easy handling with biological samples. Appropriate conditions for live cells are ensured by the microscope incubator and low exposures of light. Moreover, there is no need for specific sample preparation. The cells are just seeded into a suitable observation chamber and examined.

pdf – 1.7 MB